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This modification on the molecules will lead them to elution. In combination with the Trade of ions, ion-Trade stationary phases can easily keep certain neutral molecules. This process is connected with the retention determined by the formation of complexes, and certain ions including changeover metals is often retained on a cation-exchange resin a

-hydroxybenzoic acid—on a nonpolar C18 column employing a aqueous cellular phase that includes a buffer of acetic acid and sodium acetate. Retention situations are shorter for much less acidic cell phases simply because Each and every solute is current within an anionic, weak base type that is significantly less soluble within the nonpolar statio

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